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Coenzyme F420 is a redox cofactor involved in hydride transfer reactions in archaea and bacteria. Since F420-dependent enzymes are attracting increasing interest as tools in biocatalysis, F420 biosynthesis is being revisited. While it was commonly accepted for a long time that the 2-phospho-l-lactate (2-PL) moiety of F420 is formed from free 2-PL, it was recently shown that phosphoenolpyruvate
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Jan 15, 2021 · Resuspended extracts were vortexed and centrifuged at 10,000×g for 10 min at 4 °C, before a 0.5 ml aliquot was transferred to liquid chromatography sample vials.
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