Feb 25, 2021 · Samples were separated in 0.05 M sodium sulfate as the mobile phase, using a set of 2 x A6000M 300x8mm columns, flow rate of 1.0 mL/min, 100 μL injection volume, column/detector temperature of 25°C, and autosampler temperature of 20°C. Guluronic acid to mannuronic acid (G/M) ratio in alginate samples. Average G/M ratios for the alginates