1. Use blunt cannulas to safely transfer the SPME fibres to the conditioning vials and the samples. 2. Prepare 20 mL amber glass vials with methanol or MilliQ. (1 x 20 mL vial for 12 SPME fibres) 3. Pierce the silicone/PTFE septum of the caps with the fibres by means of the blunt cannulas 4.