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The extracts were passed through GF/F filters to remove particles and then mixed 1:1 with ammonium acetate solution (1 M). A quarter of each mixture was injected into a Shimadzu LC20A‐DAD HPLC system fitted with a 3.5‐μm C 8 column (100 × 4.6 mm
vials with glass inserts and analyzed by UHPLC–DAD–MS. UHP L C–DAD–MS analysis was conducted using a Dionex Ult imate 3000RS s y stem coupled with an Amazon SL ion trap
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1) control for arimgnzous n,ethane/acetate concentrations Head space of nitrogen end carbon dioxide, no substrate added. 2) Control for methane production: Heed space with Hydrogen end carbon dioxide as the onlu substrate. 3) Expentasotat Contains 4mM
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96 Position Round Hole Sample Rack for Oils Applications, 10 mL Vials. PerkinElmer. This 96-position sample/collection rack features wells designed for 8 mL to 13 mL vials. It's compatible with the ASX-260, ASX-280, ASX-520, ASX-520HS, ASX-560, EXR-8, XLR-8, XLR-860, and ASX-1600 and the APS-1650.
The innovative cuvette rack holds up to 12 cuvettes of 10mm light path. Features a hinged lid with secure clasp. Molded polypropylene rack includes a removable insert for easy cleaning. The height of the lid will accommodate the tallest of spectrophotometer
Difference Between 1 Ml Insulin & Tuberculin Syringe. 1 ml, Insulin Syringe are of two types – U 40 & U-100 Insulin Syringes. Iin U-40 Insulin Syringes markings on the barrel are upto 40 Units, while in U-100 markings are upto 100 Units. While in case of 1 ml Tuberculin Syringes the markings are in upto 1 ml.
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Dried metabolites were reconstituted in 100 μL of a 50% acetonitrile(ACN) 50% dH20 solution. Samples were vortexed and spun down for 10 min at 17,000g. 70 μL of the supernatant was then transferred to HPLC glass vials. 10 μL of these metabolite solutions
Typical analytical guard columns are 1 or 2 cm long with either 2.0 (2.1) or 4.0 (4.6) mm depending on the column dimensions and 1cm long for 10mm, 21.2mm & 30mm column id. Whether you opt for 1 or 2 cm long guard columns is tied to how harsh your mobile phase is and how messy your sample is.
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2020/9/1 · The order of additions for the assay was 1) Glucose (10mM), 2) Oligomycin (inhibitor of ATP synthase/complex V) (1mM), and 3) 2-deoxyglucose (2-DG) (10mM). Glucose Import: 2NBDG Uptake Assay . Glucose uptake was assayed by incubating cells with 100 μM 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG) at 37°C in glucose-free RPMI 1640 (GIBCO) containing G-5 …
