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Male reproduction in higher plants requires the support of various metabolites, including lipid molecules produced in the innermost anther wall layer (the tapetum), but how the molecules are allocated among different anther tissues remains largely unknown. Previously, rice ( Oryza sativa ) ATP binding cassette G15 (ABCG15) and its Arabidopsis ( Arabidopsis thaliana ) ortholog were shown to be
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chromatography–atmospheric pressure photoionization–mass spectrometry (LC–APPI–MS) (20–22) techniques and also two dimensional GC (GC × GC) methods are reported (23).
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